Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (B50 Neuroblastoma Cell line)
Specification
B50 Neuroblastoma Cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen Goat Anti Mouse AF555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Additional Notes
B50 Neuroblastoma cell line was grown on cover slips in 6 well plates in DMEM/10% FCS. Cells were washed with PBS prior to fixing with 1%PFA.
Wash 3x3min in PBS.
Block 10% goat serum for 20min at RT.
Primary Ab diluted 1/100 in PBS and incubated overnight at 4deg.
Wash 3x3min in PBS.
Secondary - goat anti-mouse AF555 (red) 1/1000 in PBS for 1h at RT.
Wash 3x3min in PBS
Counterstained with Hoechst 33258 (blue) for 10mins at RT.
Wash 3min in dH2O
Coverslips removed tapped dry and placed on glass slides and mounted in Invitrogen ProLong gold and kept at 4deg until imaged.
Wash 3x3min in PBS.
Block 10% goat serum for 20min at RT.
Primary Ab diluted 1/100 in PBS and incubated overnight at 4deg.
Wash 3x3min in PBS.
Secondary - goat anti-mouse AF555 (red) 1/1000 in PBS for 1h at RT.
Wash 3x3min in PBS
Counterstained with Hoechst 33258 (blue) for 10mins at RT.
Wash 3min in dH2O
Coverslips removed tapped dry and placed on glass slides and mounted in Invitrogen ProLong gold and kept at 4deg until imaged.
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提交于 Jun 21 2012