Application
Flow Cytometry
Sample
Mouse Cell (Spleen cell)
Permeabilization
No
Gating Strategy
CD45, CD11c, MHCII
Specification
Spleen cell
Preparation
Cell harvesting/tissue preparation method: Spleen smushing through 70um filter, then RBC lysis
Sample buffer: PBS + 2% FBS
Sample buffer: PBS + 2% FBS
Fixation
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + 2% FBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Used this antibody in comparison to my previous MHC II antibody (from another company) and the % MHC II+ CD11c+ cells was almost identical. Worked great!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Cheryl Sandoval
Verified customer
提交于 Jun 28 2016