Anti-MHC Class 1 H2 Db/H2-D1抗体[27-11-13] (ab25244)
Key features and details
- Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
- Suitable for: IHC-Fr, Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-MHC Class 1 H2 Db/H2-D1抗体[27-11-13]
参阅全部 MHC Class 1 H2 Db/H2-D1 一抗 -
描述
小鼠单克隆抗体[27-11-13] to MHC Class 1 H2 Db/H2-D1 -
宿主
Mouse -
特异性
ab25244 recognizes the a3 domain of H2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H2Ld, H2Dq and H2Lq, but not H2Kd or H2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed. -
经测试应用
适用于: IHC-Fr, Functional Studies, Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1. BDF1 mouse splenocytes
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表位
Recognizes an epitope on the a chain of H2Db. -
常规说明
ab25244 can be used for complement-dependent cytoxicity assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Primary antibody说明
ab25244 can be used for complement-dependent cytoxicity assays. -
克隆
单克隆 -
克隆编号
27-11-13 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
Complement-dependent cytoxicity assay. |
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. Complement-dependent cytoxicity assay. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells. -
细胞定位
Cell Membrane; single pass type I membrane protein -
数据库链接
- Entrez Gene: 14964 Mouse
- SwissProt: P01899 Mouse
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别名
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab25244 被引用在 2 文献中.
- Walsh SR et al. Type I IFN blockade uncouples immunotherapy-induced antitumor immunity and autoimmune toxicity. J Clin Invest 129:518-530 (2019). PubMed: 30422820
- Dower SK et al. The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding. J Immunol 132:751-8 (1984). PubMed: 6690617