Application
Western blot
Sample
Human Cell lysate - other (OE33 cells)
Loading amount
20 µg
Specification
OE33 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Secondary antibody
Name
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 21 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 27 2010