Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%, MOPS buffer)
Loading amount
20 µg
Specification
Mouse embryonic stem cell
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/5000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Cell Signaling goat anti-rabbit IgG, HRP-linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
LumiGlo
Exposure
30 second(s)
Bands
Specific: 130, 90 kDa
Positive control
Mouse embryonic stem cell lysate (46C)
Lane 1: Invitrogen's See Blue marker
Lane 2: mouse ES cell lysate
Additional data
Additional Notes
Gel blotted onto PVDF membrane. Lysis buffer contained complete protease inhibitor cocktail and phosSTOP phosphatase inhibitor cocktail.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 07 2012