Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Loading amount
40 µg
Specification
Muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 44 kDa Non-specific: 32, 25, 22 kDa
Additional data
Abcam response
Thank you for your valuable feedback.
To reduce the non-specific bands, the antibody can be further diluted and/or less protein can be loaded.
To reduce the non-specific bands, the antibody can be further diluted and/or less protein can be loaded.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 30 2012