Application
Western blot
Sample
Rat Tissue lysate - whole (Skin)
Loading amount
15 µg
Specification
Skin
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: monoclonal anti Rabbit
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
BCIP/NBT
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
Ran 2 samples in duplicate each with different treatment and all showed bands around 45kDa
Additional data
Additional Notes
Used a PVDF membrane, Primary was incubated overnight at 4 degrees C.
Antibodies were diluted in 1.5% TBS-T
Antibodies were diluted in 1.5% TBS-T
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 20 2006