Anti-ME1抗体(ab97445)
Key features and details
- Rabbit polyclonal to ME1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ME1抗体
参阅全部 ME1 一抗 -
描述
兔多克隆抗体to ME1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97445于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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组织特异性
Expressed in all tissues tested including liver, placenta and white adipose tissue. -
序列相似性
Belongs to the malic enzymes family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4199 Human
- Entrez Gene: 17436 Mouse
- Entrez Gene: 24552 Rat
- Omim: 154250 Human
- SwissProt: P48163 Human
- SwissProt: P06801 Mouse
- SwissProt: P13697 Rat
- Unigene: 21160 Human
see all -
别名
- Cytosolic malic enzyme 1 antibody
- HUMNDME antibody
- Malate dehydrogenase antibody
see all
图片
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All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
Lower image: merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab97445 被引用在 21 文献中.
- Jiang Z et al. miR-885-5p Inhibits Invasion and Metastasis in Gastric Cancer by Targeting Malic Enzyme 1. DNA Cell Biol 40:694-705 (2021). PubMed: 33751897
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Li H et al. CMap analysis identifies Atractyloside as a potential drug candidate for type 2 diabetes based on integration of metabolomics and transcriptomics. J Cell Mol Med 24:7417-7426 (2020). PubMed: 32469143
- Shao C et al. Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. Redox Biol 36:101685 (2020). PubMed: 32853879
- Ghergurovich JM et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol 16:731-739 (2020). PubMed: 32393898