Application
Western blot
Sample
Mouse Cell lysate - whole cell (Eu-Myc Arf-/- mouse lymphoma cells)
Loading amount
30 µg
Specification
Eu-Myc Arf-/- mouse lymphoma cells
Treatment
RNAi knockdown
Gel Running Conditions
Reduced Denaturing (5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 190 kDa
Positive control
Cell lysates of vector infected cells
Negative control
lysates of MDC1 knockdown cells.
Additional data
Additional Notes
Signal was very strong.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Hai Jiang
Verified customer
提交于 Jun 09 2008