Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 0.1% TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Li-CoR Goat anti Rabbit Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey Scanner
Bands
Specific: ~255, ~ 270, ~150 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
1/1000 dilution gives very weak signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 31 2014