Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal Cord)
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Pierce Stabilized Goat anti-Rabbit Conjugated to H
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 24 kDa
Additional data
Additional Notes
We used ab25124 on whole rat (or mouse lane 2) spinal cord protein lysate. We loaded 40 ug of total protein, (confirmed with B-actin loading control ab6276.) I ran two gels and incubated one with 1/2000 and one 1/1000 dilution of the primary antibody (ab25124). The 1/1000 dilution looked much better in our hands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Nancy Nutile-McMenemy
Verified customer
提交于 Dec 07 2005