Application
Western blot
Sample
Human Recombinant protein (Recombinant protein)
Loading amount
0.2 µg
Specification
Recombinant protein
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tween-TBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 11, 18, 35 kDa
Additional data
Additional Notes
lane1: 0.2ug/lane rhMCP-1
lane2: 0.0125ug/lane rhMCP-1
Used rhMCP-1 was purchased from Peprotech (cat#300-04),
which molecular weight is an 8.6kDa (76 amino acid).
When I reconstitute rhMCP-1, I added BSA for stabilizing rhMCP-1.
The band at 11kDa may be a rhMCP-1.
lane2: 0.0125ug/lane rhMCP-1
Used rhMCP-1 was purchased from Peprotech (cat#300-04),
which molecular weight is an 8.6kDa (76 amino acid).
When I reconstitute rhMCP-1, I added BSA for stabilizing rhMCP-1.
The band at 11kDa may be a rhMCP-1.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 17 2010