Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labelled and counterstained with DAPI. Under these conditions, the antibody did not produce the expected labeling pattern. MeOH fixed samples were also evaluated and did not produce the expected pattern.
Abcam response
Thank you for your valuable feedback. ab76023 has not been previously tested in ICC/IF. Your data suggests that ab76023 may not be suitable to detect MCM5 in HeLa cells under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Jan 02 2018