Anti-MCM4 (phospho S54)抗体(ab74014)
Key features and details
- Rabbit polyclonal to MCM4 (phospho S54)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
概述
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产品名称
Anti-MCM4 (phospho S54)抗体
参阅全部 MCM4 一抗 -
描述
兔多克隆抗体to MCM4 (phospho S54) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: African green monkey
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human MCM4 (phospho S54).
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阳性对照
- Extracts from COS-7 cells treated with nocodazole (1uM, 16hours).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74014于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 96 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 96 kDa. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 17217 Mouse
- SwissProt: P49717 Mouse
- Unigene: 1500 Mouse
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别名
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
图片
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All lanes : Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours)
Lane 2 : Extracts from COS-7 cells
treated with nocodazole (1uM, 16hours) with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 96 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab74014 尚未被引用在任何文献中。