Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
100 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk/PBS/0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse IgG (Whole Molecule) Peroxidase Conjuga
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 125 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 18 2008