Anti-MCAK抗体(ab71706)
Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-MCAK抗体
参阅全部 MCAK 一抗 -
描述
兔多克隆抗体to MCAK -
宿主
Rabbit -
特异性
Does not react with human MCAK. -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Mouse MCAK. A region between residue 50 and 100 of mouse MCAK. GenBank: NP_608301.2
Database link: Q922S8 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71706于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
组织特异性
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
序列相似性
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
发展阶段
Isoform 2 is expressed in fetal testis. -
结构域
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
细胞定位
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
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数据库链接
- Entrez Gene: 73804 Mouse
- Entrez Gene: 171529 Rat
- SwissProt: Q922S8 Mouse
- SwissProt: Q62909 Rat
- Unigene: 247651 Mouse
- Unigene: 219966 Rat
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别名
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
图片
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All lanes : Anti-MCAK antibody (ab71706) at 0.1 µg/ml
Lane 1 : NIH3T3 whole cell lysate at 50 µg/ml
Lane 2 : NIH3T3 whole cell lysate at 15 µg/ml
Lane 3 : NIH3T3 whole cell lysate at 5 µg/ml
Predicted band size: 81 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse hybridoma tumor tissue labelling MCAK with ab71706 at 1/1000 (1µg/ml). Detection: DAB.
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MCAK antibody (ab71706) recognizing MCAK protein in NIH3T3 whole cell lysate (1 mg, 20% of IP loaded).
Lane 1: MCAK antibody (ab71706) used for IP at 3 µg/mg lysate and for WB at 1.0 µg/ml.
Lane 2: Non-Abcam MCAK antibody, specific for a downstream epitope of MCAK.
Lane 3: Control IgG.
Detection by chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab71706 被引用在 1 文献中.
- Huang CH et al. Identification of aberrantly methylated differentially expressed genes and pro-tumorigenic role of KIF2C in melanoma. Front Genet 13:817656 (2022). PubMed: 35991567