Anti-MAX抗体(ab101271)
Key features and details
- Rabbit polyclonal to MAX
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MAX抗体
参阅全部 MAX 一抗 -
描述
兔多克隆抗体to MAX -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide within Human MAX aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P61244 -
阳性对照
- HeLa and 293T whole cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human ovarian tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 18 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
组织特异性
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
疾病相关
Pheochromocytoma -
序列相似性
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
翻译后修饰
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
细胞定位
Nucleus. Cell projection, dendrite. - Information by UniProt
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数据库链接
- Entrez Gene: 4149 Human
- Entrez Gene: 17187 Mouse
- Omim: 154950 Human
- SwissProt: P52162 Chicken
- SwissProt: P61244 Human
- SwissProt: P28574 Mouse
- Unigene: 285354 Human
- Unigene: 268548 Mouse
see all -
别名
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAX with ab101271 at 1/1000 (1µg/ml).
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All lanes : Anti-MAX antibody (ab101271) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes -
Detection of MAX in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101271 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents Immunoprecipitates from control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab101271 被引用在 4 文献中.
- Yamashita T et al. Decreased MYC-associated factor X (MAX) expression is a new potential biomarker for adverse prognosis in anaplastic large cell lymphoma. Sci Rep 10:10391 (2020). PubMed: 32587329
- Chang X et al. A Novel Phenotype of Germline Pathogenic Variants in MAX: Concurrence of Pheochromocytoma and Ganglioneuroma in a Chinese Family and Literature Review. Front Endocrinol (Lausanne) 11:558 (2020). PubMed: 32973681
- Nicolson NG et al. Transcription Factor Profiling Identifies Spatially Heterogenous Mediators of Follicular Thyroid Cancer Invasion. Endocr Pathol 31:367-376 (2020). PubMed: 33063251
- Malchenko S et al. Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS One 12:e0173106 (2017). IHC-P ; Human . PubMed: 28249000