Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cysts)
Permeabilization
Yes - Tritón 0.3 %
Specification
MDCK cysts
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 16°C
Fixative
Methanol
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: PBS/BSA 1%/Tritón 0.3%
Dilution
1/400
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Abcam response
Thank you for participating in our Abtrial and your valuable feedback. We are happy to see that this antibody worked well for you in dog, a species that has not been tested before. We welcome any further feedback and Abreviews from other customers who have used this antibody in dog which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. M. Cecilia Larocca
Verified customer
提交于 Jan 28 2016