Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
Yes - 50% Ethanol 30 min
Fixative
Paraformaldehyde
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+Triton X-100 0.3%
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Life Technologies anti-chicken IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Positivity also good at 1:500 dilution, but rather dim at 1:1000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Tom Owen
Verified customer
提交于 Apr 08 2015