Anti-MAGEA2抗体- Aminoterminal end (ab71786)
Key features and details
- Rabbit polyclonal to MAGEA2 - Aminoterminal end
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MAGEA2抗体- Aminoterminal end
参阅全部 MAGEA2 一抗 -
描述
兔多克隆抗体to MAGEA2 - Aminoterminal end -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A KLH conjugated synthetic peptide selected from the N terminal region of human MAGEA2.
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阳性对照
- Human breast carcinoma tissue and HL60 lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71786于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
IHC-P |
说明 |
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IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 35 kDa plus many other bands (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation. -
组织特异性
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. -
序列相似性
Contains 1 MAGE domain. -
细胞定位
Nucleus. Nucleus, PML body. - Information by UniProt
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数据库链接
- Entrez Gene: 266740 Human
- Entrez Gene: 4101 Human
- Omim: 300173 Human
- Omim: 300549 Human
- SwissProt: P43356 Human
- Unigene: 534597 Human
- Unigene: 713061 Human
- Unigene: 736992 Human
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别名
- Cancer/testis antigen 1.2 antibody
- Cancer/testis antigen family 1, member 2 antibody
- CT1.2 antibody
see all
图片
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Anti-MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution + HL60 lysate at 12.5 µg
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
数据表及文件
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Datasheet download
文献 (0)
ab71786 尚未被引用在任何文献中。