Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (primary alveolar macrophage)
Permeabilization
Yes - TX-100
Specification
primary alveolar macrophage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS
Dilution
1/150
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: goat anti rabbit Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
The antibody worked both on paraformaldehyde and methanol fixed samples.
Picture represents 1 macrophage.
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提交于 Feb 05 2009