Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS-0.05%saponin
Secondary antibody
Name
Non-Abcam antibody was used: donkey-anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Strong nuclear background
Abcam response
This Review was investigated by the Scientific Support team. MCF-7 cells were recommended as positive control, and longer incubation times for the antibody.
Also, different fixatives such as ice-cold acetone, or methanol or ethanol for 5 min (not longer than 10 min) were suggested. A blocking agent like BSA could reduce the background signal.
Unfortunately, the protocol used for this review has proved not to be suitable for this antibody.
Also, different fixatives such as ice-cold acetone, or methanol or ethanol for 5 min (not longer than 10 min) were suggested. A blocking agent like BSA could reduce the background signal.
Unfortunately, the protocol used for this review has proved not to be suitable for this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 30 2012