Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-EDTA Buffer
Sample
Mouse Tissue sections (skin wound healing tissue)
Specification
skin wound healing tissue
Permeabilization
Yes - 0.1% triton-X100 for 10 min before blocking step
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBS pH 7.6
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG(H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 16 2014