Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Loading amount
50 µg
Specification
HEK-293
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: tbst + 0.1% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Pierce Supersignal West Pico
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 56 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 01 2010