Anti-LTB抗体(ab64835)
Key features and details
- Rabbit polyclonal to LTB
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-LTB抗体
参阅全部 LTB 一抗 -
描述
兔多克隆抗体to LTB -
宿主
Rabbit -
特异性
ab64835 detects endogenous levels of total Lymphotoxin Beta protein. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human LTB (C terminal).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500 - 1/1000.
|
|
WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
|
说明 |
---|
ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
靶标
-
功能
Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. -
组织特异性
Spleen and thymus. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 4050 Human
- Omim: 600978 Human
- SwissProt: Q06643 Human
- Unigene: 376208 Human
-
别名
- LT beta antibody
- LT-beta antibody
- Ltb antibody
see all
图片
-
All lanes : Anti-LTB antibody (ab64835) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 25 kDa
Observed band size: 25 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab64835 被引用在 7 文献中.
- Morelli M et al. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics. Methods Mol Biol 2248:155-165 (2021). PubMed: 33185874
- Fania L et al. Paradoxical psoriasis induced by TNF-a blockade shows immunological features typical of the early phase of psoriasis development. J Pathol Clin Res 6:55-68 (2020). PubMed: 31577850
- Francipane MG et al. Host Lymphotoxin-ß Receptor Signaling Is Crucial for Angiogenesis of Metanephric Tissue Transplanted into Lymphoid Sites. Am J Pathol 190:252-269 (2020). PubMed: 31585070
- Cortes-Selva D et al. IL-4 promotes stromal cell expansion and is critical for development of a type-2, but not a type 1 immune response. Eur J Immunol 49:428-442 (2019). PubMed: 30575951
- Yim RL et al. Methylation of miR-155-3p in mantle cell lymphoma and other non-Hodgkin's lymphomas. Oncotarget 5:9770-82 (2014). Flow Cyt . PubMed: 25211095
- Simonin Y et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog 9:e1003234 (2013). WB, IHC-P ; Mouse . PubMed: 23555249
- Watanabe T et al. Higher-order chromatin regulation and differential gene expression in the human tumor necrosis factor/lymphotoxin locus in hepatocellular carcinoma cells. Mol Cell Biol 32:1529-41 (2012). IHC-P ; Human . PubMed: 22354988