Liver Carboxylesterase 1 overexpression 293T裂解物(whole cell) (ab94047)
概述
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产品名称
Liver Carboxylesterase 1 overexpression 293T裂解物(whole cell)
参阅全部 Liver Carboxylesterase 1/CES1 细胞裂解液 -
常规说明
ab94047 is a 293T cell transfected lysate in which Human Liver Carboxylesterase 1 has been transiently over-expressed using a pCMV-Liver Carboxylesterase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. PTM: Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. Similarity: Belongs to the type-B carboxylesterase/lipase family. Tissue specificity: Expressed predominantly in liver with lower levels in heart and lung.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94047于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94047 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab89779) at 1/500 dilution
Lane 1 :Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94047 尚未被引用在任何文献中。