重组Anti-Lipoamide Dehydrogenase抗体[EPR6634(B)] (ab124926)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6634(B)] to Lipoamide Dehydrogenase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Lipoamide Dehydrogenase抗体[EPR6634(B)]
参阅全部 Lipoamide Dehydrogenase 一抗 -
描述
兔单克隆抗体[EPR6634(B)] to Lipoamide Dehydrogenase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Lipoamide Dehydrogenase aa 50-150. The exact sequence is proprietary.
-
阳性对照
- WB: Jurkat, MCF7, HeLa, and 293T cell lysates. IHC-P: Human colon tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 8.50 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6634(B) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa).
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. -
疾病相关
Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. -
序列相似性
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
翻译后修饰
Tyrosine phosphorylated. -
细胞定位
Mitochondrion matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 1738 Human
- Entrez Gene: 13382 Mouse
- Entrez Gene: 298942 Rat
- Omim: 238331 Human
- SwissProt: P09622 Human
- SwissProt: O08749 Mouse
- SwissProt: Q6P6R2 Rat
- Unigene: 131711 Human
see all -
别名
- Dehydrogenase complex, E3 component antibody
- Diaphorase antibody
- Dihydrolipoamide dehydrogenase antibody
see all
图片
-
All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] (ab124926) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DLD knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab124926 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124926 Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] was shown to specifically react with Lipoamide Dehydrogenase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265535 (knockout cell lysate ab257922) was used. Wild-type and Lipoamide Dehydrogenase knockout samples were subjected to SDS-PAGE. ab124926 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
ab124926 at 1/100 dilution staining Lipoamide Dehydrogenase in Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-Lipoamide Dehydrogenase antibody [EPR6634(B)] (ab124926) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab124926 被引用在 1 文献中.
- Malty RH et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). PubMed: 29128334