Anti-Lipin 1抗体(ab70138)
Key features and details
- Rabbit polyclonal to Lipin 1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Lipin 1抗体
参阅全部 Lipin 1 一抗 -
描述
兔多克隆抗体to Lipin 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee -
免疫原
Synthetic peptide corresponding to Human Lipin 1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab86631) -
阳性对照
- This antibody gave a positive signal in the following Mouse Tissue Lysates: Liver; Pancreas, Kidney.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70138于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
靶标
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功能
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. -
组织特异性
Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract. -
疾病相关
Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years. -
序列相似性
Belongs to the lipin family. -
结构域
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity. -
翻译后修饰
Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
Sumoylated. -
细胞定位
Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23175 Human
- Entrez Gene: 14245 Mouse
- Entrez Gene: 313977 Rat
- Omim: 605518 Human
- SwissProt: Q14693 Human
- SwissProt: Q91ZP3 Mouse
- Unigene: 467740 Human
- Unigene: 153625 Mouse
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别名
- EC=3.1.3.4 antibody
- KIAA0188 antibody
- Lipin-1 antibody
see all
图片
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All lanes : Anti-Lipin 1 antibody (ab70138) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab70138 被引用在 6 文献中.
- Cui ZY et al. Allium victorialis L. Extracts Promote Activity of FXR to Ameliorate Alcoholic Liver Disease: Targeting Liver Lipid Deposition and Inflammation. Front Pharmacol 12:738689 (2021). PubMed: 34690775
- Guo Z et al. mTORC2 Regulates Lipogenic Gene Expression through PPAR? to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int 2019:5196028 (2019). PubMed: 31223619
- Lin YC et al. Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30934807
- Lizardo K et al. Diet Alters Serum Metabolomic Profiling in the Mouse Model of Chronic Chagas Cardiomyopathy. Dis Markers 2019:4956016 (2019). PubMed: 31949545
- Zhang W et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury. Sci Rep 8:408 (2018). WB ; Mouse . PubMed: 29323242
- Cai K et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014). WB . PubMed: 24369117