Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Permeabilization
Yes - 0.1% triton X-100
Specification
embryonic stem cell
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Fixative
methanol:acetone 1:1
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: block
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: alexa fluor goat anti-Rabbit IgG 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Blue staining is DAPI, green is Lin28. This staining was performed using methanol/acetone fixation, however PFA fixation resulted in very diffuse staining across the whole cell rather than entirely nuclear as depicted here.
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提交于 Aug 14 2012