Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - 0.1% Tween in TBS
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Modified Davidson's Fixative
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS- 0.3% Triton X
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector Biotinylated anti-rabbit IgG (H+L) Conjugat
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Mouse ovaries (age 7 weeks) were fixed in Modified Davidson's Fixative, embedded in paraffin and processed for antigen retrieval using heat and sodium Lin28 antibody (ab46020) overnight at 4C at 1:100 dilution. The antibody shows staining in predominantly in oocytes and mural granulosa cells and at low levels in stromal, thecal, and the ovarian surface epithelium.
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提交于 Jan 18 2012