Anti-Enkephalin/ENK抗体(ab85798)
Key features and details
- Rabbit polyclonal to Enkephalin/ENK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Enkephalin/ENK抗体
参阅全部 Enkephalin/ENK 一抗 -
描述
兔多克隆抗体to Enkephalin/ENK -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Enkephalin/ENK aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102738) -
阳性对照
- This antibody gave a positive signal in Human testis tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). |
靶标
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功能
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
序列相似性
Belongs to the opioid neuropeptide precursor family. -
翻译后修饰
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5179 Human
- Entrez Gene: 18619 Mouse
- Entrez Gene: 29237 Rat
- Omim: 131330 Human
- SwissProt: P01210 Human
- SwissProt: P22005 Mouse
- SwissProt: P04094 Rat
- Unigene: 339831 Human
see all -
别名
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
see all
图片
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Anti-Enkephalin/ENK antibody (ab85798) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThe band observed at 28 kDa could potentially be a cleaved form of Enkephalin due to the presence of a 24 amino acid signal peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab85798 被引用在 3 文献中.
- Cai H et al. Inhibition of endoplasmic reticulum stress reverses synaptic plasticity deficits in striatum of DYT1 dystonia mice. Aging (Albany NY) 13:20319-20334 (2021). PubMed: 34398825
- Creus-Muncunill J et al. Huntington's disease brain-derived small RNAs recapitulate associated neuropathology in mice. Acta Neuropathol 141:565-584 (2021). PubMed: 33547932
- Bulc M et al. Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes. J Diabetes Res 2018:4735659 (2018). PubMed: 30140706