Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cell)
Specification
Mesenchymal stem cell
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 10% horse serum
Secondary antibody
Name
Non-Abcam antibody was used: Southern Biotech IgG1 Mouse anti rabbit
Host species: Mouse
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Mouse
Clonality: Monoclonal
Conjugation: Fluorescein
Dilution
1/100
Additional data
Additional Notes
Good quality antibody used in the immunofluorescent staining of bone marrow and lamina propria derived mesenchymal stem cells. Staining was punctate, and vibrantly showed up the outline of the cells. Staining was reproducable over multiple replicates from both cell types.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Steven Johnstone
Verified customer
提交于 Sep 05 2012