Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone marrow)
Permeabilization
Yes - 0.025% Triton X
Specification
Bone marrow
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% normal donkey serum
Concentration
5 µg/ml
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor 647 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Abcam response
Thank you so much for testing this antibody in IHC-F and mouse. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species and application.
The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 18 2017