Anti-Laminin gamma 1抗体[A5] - BSA and Azide free (ab80580)

概述

  • 产品名称
    Anti-Laminin gamma 1抗体[A5] - BSA and Azide free
    参阅全部 Laminin gamma 1 一抗
  • 描述
    大鼠单克隆抗体[A5] to Laminin gamma 1 - BSA and Azide free
  • 特异性
    This antibody uniformly stains all human and murine basement membranes.
  • 经测试应用
    适用于: IP, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Murine EHS laminin preparation.

  • 阳性对照
    • LS174T cells. Normal colon or colon carcinoma.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

应用

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
IHC-P
IHC-Fr
ICC/IF
  • 应用说明
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml.
    IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
    IP: Use at 2µg/mg of lysate. (Native and denatured)

    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • 组织特异性
      Found in the basement membranes (major component).
    • 序列相似性
      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • 结构域
      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    图片

    • Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
    • Native IP using LS174T cells:
      IP 2µg/mg lysate.
      WB at 2µg/ml.

    文献

    This product has been referenced in:
    • Calyjur PC  et al. The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS One 11:e0150748 (2016). IHC . Read more (PubMed: 26954670) »
    • Ljubimov AV  et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 7723285) »

    See all 4 Publications for this product

    客户评价及客户问答

    Thank you for contacting us. We can recommend using antibody ab106783, ab99667, ab99666 etc. Rabbit or goat anti rat IgG also cross reacts with IgG2a so anti rat IgGs can also be used. I hope this information is helpful to you. Please do not hesitate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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