Anti-Laminin抗体(ab11575)
Key features and details
- Rabbit polyclonal to Laminin
- Suitable for: Dot blot, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Laminin抗体
参阅全部 Laminin 一抗 -
描述
兔多克隆抗体to Laminin -
宿主
Rabbit -
特异性
In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C. -
经测试应用
适用于: Dot blot, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Dog, Pig, Xenopus laevis, Mammals, Amphibian -
免疫原
Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
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常规说明
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.027% PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Dot blot |
1/1000. This concentration was determined using laminin at 50 ng per dot.
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IHC-P | (27) |
Use at an assay dependent concentration.
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说明 |
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Dot blot
1/1000. This concentration was determined using laminin at 50 ng per dot. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
组织特异性
Broadly expressed in: skin, heart, lung, and the reproductive tracts. -
序列相似性
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10319 Human
- Entrez Gene: 284217 Human
- Entrez Gene: 3908 Human
- Entrez Gene: 3912 Human
- Entrez Gene: 3913 Human
- Entrez Gene: 3915 Human
- Entrez Gene: 3918 Human
- Entrez Gene: 16772 Mouse
see all -
别名
- LAMA 1 antibody
- LAMA antibody
- LAMA1 antibody
see all
图片
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Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
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Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.
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Immunoblotting
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
Lanes
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control: without primary antibody -
This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (567)
ab11575 被引用在 567 文献中.
- Mesquita FCP et al. Decellularized Extracellular Matrix Powder Accelerates Metabolic Maturation at Early Stages of Cardiac Differentiation in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cells Tissues Organs 212:32-44 (2023). PubMed: 34933302
- Song MJ et al. Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration. Nat Methods 20:149-161 (2023). PubMed: 36550275
- Adegoke MA et al. Flexibility of in vitro cortical circuits influences resilience from microtrauma. Front Cell Neurosci 16:991740 (2022). PubMed: 36589287
- Xu J et al. Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling. Am J Cancer Res 12:5552-5563 (2022). PubMed: 36628278
- Rostami S et al. Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite Allotransplantation. Transplant Direct 8:e1251 (2022). PubMed: 34912943