Application
Immunoprecipitation
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Total protein in input
5e+006 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
Canine Kidney Epithelial MDCK cells
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/500
Western Blot antibody
Dilution
1/1000
Name
Abcam antibody:Anti-Lamin B1 antibody - Nuclear Envelope Marker
Additional data
Additional Notes
Lane 1: IP by control rabbit IgG (Abcam ab27472)
Lane 2: IP by Lamin B1 antibody (Abcam ab16048).
2ug of each antibody was used for IP experiments.
Band corresponding to Lamin B1 (70kDa) can be detected in Lamin B1 antibody pulldown sample but not control IgG sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 04 2016