Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116, colon cancer)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
60 µg
Treatment
24 hrs, different treatment
Specification
HCT116, colon cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in PBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Pierce Goat Anti-Mouse IgG, (H+L) Peroxidase Conju
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL with 10% ECL+
Exposure
30 second(s)
Bands
Specific: 75, 70 kDa
Additional data
Additional Notes
Really an excellent antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Christian Marx
Verified customer
提交于 Aug 21 2012