Lactate Dehydrogenase (LDH) Assay试剂盒(Fluorometric) (ab197000)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Adherent cells, Cell culture media, Other biological fluids, Plasma, Serum, Suspension cells, Tissue Extracts, Urine
- Sensitivity: 1 uU/ml
概述
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产品名称
Lactate Dehydrogenase (LDH) Assay试剂盒(Fluorometric)
参阅全部 Lactate Dehydrogenase 试剂盒 -
检测方法
Fluorescent -
样品类型
Urine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media -
检测类型
Enzyme activity -
灵敏度
1 uU/ml -
检测时间
0h 20m -
种属反应性
与反应: Mammals, Other species -
产品概述
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).
This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K730 PicoProbe™ Lactate Dehydrogenase Activity Assay Kit. K730-500 is the same size as the 500 test size of ab197000.
Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests 500 tests Developer Mix I 1 vial 1 vial LDH Assay Buffer 1 x 110ml 1 x 110ml LDH Positive Control 1 vial 1 vial NADH Standard I 1 vial 1 vial PicoProbe (1.5 ml) 1 x 1.4ml 1 x 1.4ml -
研究领域
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通路
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
疾病相关
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
序列相似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
数据库链接
- SwissProt: Q7SI97 Other species
相关产品
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Assay kits
图片
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Typical NADH Standard Curve obtained following assay protocol.
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Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.
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Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab197000 被引用在 13 文献中.
- Pyun J et al. Copper bis(thiosemicarbazone) complexes modulate P-glycoprotein expression and function in human brain microvascular endothelial cells. J Neurochem 162:226-244 (2022). PubMed: 35304760
- Nimiritsky P et al. Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism. Biomedicines 9:N/A (2021). PubMed: 34572378
- Visavadiya NP et al. Distinct glycolytic pathway regulation in liver, tumour and skeletal muscle of mice with cancer cachexia. Cell Biochem Funct 39:802-812 (2021). PubMed: 34129243
- Zhao Y et al. Clinical features of cardiac lymphoma: an analysis of 37 cases. J Int Med Res 49:300060521999558 (2021). PubMed: 33752450
- Jose A et al. Anti-proliferative potential of phytochemical fractions isolated from Simarouba glauca DC leaf. Heliyon 6:e03836 (2020). PubMed: 32373740