Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse embryo, E14.5)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Mouse embryo, E14.5
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa-Fluor 488 goat-anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
E14.5 mouse embryos fixed in 4% PFA, paraffin-embedded, cross sections. Image shows lymphatic vessels just under embryo skin outlined in green. Tissue is also stained for Prox-1 (Non-Abcam antibody) in red (Alexa-goat-anti-mouse 594). Nuclei stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Molly Plehaty
Verified customer
提交于 Sep 15 2016