Application
Flow Cytometry
Sample
Rat Cell (Skin, lymphatic endothelial cells)
Permeabilization
No
Gating Strategy
Single lymphatic endothelial cells (area: lymphocytes) dead cells and debris were excluded
Specification
Skin, lymphatic endothelial cells
Preparation
Cell harvesting/tissue preparation method: Digestion in collagenase 1 + DNase I solution.
Sample buffer: PBS + 2mM EDTA + 2%FCS
Sample buffer: PBS + 2mM EDTA + 2%FCS
Fixation
Not fixed, live cells
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS+ 2mM EDTA+ 2%FCS
Concentration
1 µg/cells
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor 647
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor 647
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提交于 Aug 04 2017