Anti-LYVE1抗体(ab10278)
Key features and details
- Rabbit polyclonal to LYVE1
- Suitable for: IHC-Fr, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LYVE1抗体
参阅全部 LYVE1 一抗 -
描述
兔多克隆抗体to LYVE1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
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阳性对照
- human colon carcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Protein-A Chromatography (+his tag depleted). -
Primary antibody说明
The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, podoplanin. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use a concentration of 6 - 30 µg/ml.
Fix sections for 10 min at -20°C in MeOH. |
Flow Cyt | (1) |
Use at an assay dependent concentration.
|
IHC-P | (2) |
Use a concentration of 2 µg/ml.
|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35-45 kDa (predicted molecular weight: 35 kDa).
|
说明 |
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IHC-Fr
Use a concentration of 6 - 30 µg/ml. Fix sections for 10 min at -20°C in MeOH. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35-45 kDa (predicted molecular weight: 35 kDa). |
靶标
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功能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
组织特异性
Mainly expressed in endothelial cells lining lymphatic vessels. -
序列相似性
Contains 1 Link domain. -
翻译后修饰
O-glycosylated. -
细胞定位
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 10894 Human
- Entrez Gene: 293186 Rat
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
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别名
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
图片
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Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.
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ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
All lanes : Anti-LYVE1 antibody (ab10278) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
LYVE-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.
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Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab10278 被引用在 13 文献中.
- Michalak-Micka K et al. Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin. Biomedicines 10:N/A (2022). PubMed: 35327478
- Pontiggia L et al. Bioprinting and plastic compression of large pigmented and vascularized human dermo-epidermal skin substitutes by means of a new robotic platform. J Tissue Eng 13:20417314221088513 (2022). PubMed: 35495096
- Li J et al. ZKSCAN5 Activates VEGFC Expression by Recruiting SETD7 to Promote the Lymphangiogenesis, Tumour Growth, and Metastasis of Breast Cancer. Front Oncol 12:875033 (2022). PubMed: 35600335
- Wu S et al. A Dual Targeting Magnetic Nanoparticle for Human Cancer Detection. Nanoscale Res Lett 14:228 (2019). PubMed: 31289961
- Li P et al. Clinical significance of CCBE1 expression in lung cancer. Mol Med Rep 17:2107-2112 (2018). PubMed: 29207117