Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
A431 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Fempto 30%
Exposure
4 second(s)
Bands
Specific: ~52 kDa Non-specific: 130, 80, 58, 50 kDa
Additional data
Additional Notes
Lane 1 - LXR alpha and beta KD
Lane 2 - control
Lane 3 - LXR alpha only KD
Lane 4 - LXR beta KD
Lane 5 - LXR alpha and beta KD
The correct band is identified by the missing bands in the LXR beta KD samples
Abcam response
Thank you for sharing your feedback with this review. You may reduce the background bands by blocking for longer or reducing the incubation time with primary antibody to 1-2 hours at room temperature. Please note our Abpromise guarantees the antibody for tested applications and species for 12 months from purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 18 2015