Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (6% Gel)
Loading amount
3.25 µg
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in 0.1% TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/30000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) (Cat# 31460)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~140 kDa Non-specific: ~85 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 26 2015