重组Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503)抗体[EP2360Y] (ab76417)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490) + LRP5 (phospho S1503)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503)抗体[EP2360Y] -
描述
兔单克隆抗体[EP2360Y] to LRP6 (phospho S1490) + LRP5 (phospho S1503) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysates treated with Calyculin, 293T transfected with LRP6 and LRP5 overexpression vector whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2360Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76417于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 179 kDa.
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说明 |
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WB
1/500 - 1/10000. Predicted molecular weight: 179 kDa. |
靶标
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细胞定位
LRP6: Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD. LRP5: Membrane. Endoplasmic reticulum. Chaperoned to the plasma membrane by MESD. -
数据库链接
- Entrez Gene: 4040 Human
- Entrez Gene: 4041 Human
- Omim: 603506 Human
- Omim: 603507 Human
- SwissProt: O75197 Human
- SwissProt: O75581 Human
- Unigene: 584775 Human
- Unigene: 6347 Human
see all
图片
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Lanes 1-3 : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/10000 dilution
Lanes 4-6 : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/10000 dilution
Lanes 1 & 4 : 293T transfected with blank vector whole cell lysate
Lanes 2 & 5 : 293T transfected with LRP6 overexpression vector whole cell lysate
Lanes 3 & 6 : 293T transfected with LRP5 overexpression vector whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5%NFDM/TBST.
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All lanes : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/1000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell
lysates treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 179 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76417 被引用在 4 文献中.
- Sun Y et al. CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo. J Gene Med 24:e3343 (2022). PubMed: 33871149
- Miao J et al. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells. Physiol Rep 9:e14696 (2021). PubMed: 33463897
- Sun Y et al. LncRNA H19/miR-194/PFTK1 axis modulates the cell proliferation and migration of pancreatic cancer. J Cell Biochem 120:3874-3886 (2019). PubMed: 30474270
- Shi Z et al. Novel Mutation of LRP6 Identified in Chinese Han Population Links Canonical WNT Signaling to Neural Tube Defects. Birth Defects Res 110:63-71 (2018). PubMed: 28960852