Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Astrocytes culture)
Specification
Rat Astrocytes culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/200
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 0.1% TritonX with 0.1x PBS- 10% Donkeys
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluro 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Post natal day three (P3) rat astrocytes (mixed glia) grew in DMEM plus 10% FCS. Microglia was separated from confluent cells by shaking for two hours and then grew as Astrocytes culture. The cells were fixed with 4% PFA in 0.1M PBS. Immunofuorescent staining performed with anti-LRP1 (ab92544) with Alexa 488. Nucleus stained with Hoechst at high power magnification. Very good expression was seen in the astrocytes membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Dec 14 2012