Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor 555) preadsorbed
Additional data
Additional Notes
LOXL2 should be found in early differentiating spermatogonia, but become undetectable starting in spermatocytes. This antibody appears to stain all stages of germ cells with high backround noise and seems to be brightest in spermatocytes. DAPI (blue) was used as a counterstain to label nuclei.
Abcam response
Thank you for sharing this data with us. Previously ab96233 has not been tested in IHC-Fr. Based on the data shown here, ab96233 does not work in IHC-Fr, under these experimental conditions. We welcome feedback from other researchers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
提交于 Jan 31 2018