Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart and carotid artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Retrieval sol. H-3300 Vector lab
Permeabilization
No
Specification
heart and carotid artery
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
formalin 10% buffered neutral
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit-ALEXA IgG (H+L) (LifeTechnology A
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
1st antibody incubated for over night at 4°C or for 4 hours at RT
Conjugation of 2nd antibody is correctly Alexa Fluor 633 nm (was not offered as a chose)
Used TrisBuffer with Tween20
Antigen Retrieval was in water bath 20' at 98°C
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提交于 Oct 21 2014