重组Anti-LMO2抗体[EP3257] (ab91652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3257] to LMO2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-LMO2抗体[EP3257]
参阅全部 LMO2 一抗 -
描述
兔单克隆抗体[EP3257] to LMO2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human LMO2. The exact sequence is proprietary.
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阳性对照
- WB: Raji and HEK-293 cell lysate and Fetal kidney, mouse E14 embryo, and rat E14 embryo tissue lysate IP: Raji whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3257 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91652于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
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|
IP |
1/30.
For unpurified use at 1/100 - 1/500 |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
IP
1/30. For unpurified use at 1/100 - 1/500 |
靶标
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功能
Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state. -
疾病相关
Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD. -
序列相似性
Contains 2 LIM zinc-binding domains. -
结构域
The second LIM zinc-binding domain interacts with KDM5A. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4005 Human
- Entrez Gene: 16909 Mouse
- Entrez Gene: 362176 Rat
- Omim: 180385 Human
- SwissProt: P25791 Human
- SwissProt: P25801 Mouse
- Unigene: 34560 Human
- Unigene: 29266 Mouse
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别名
- Cysteine rich protein TTG 2 antibody
- Cysteine rich protein TTG2 antibody
- Cysteine-rich protein TTG-2 antibody
see all
图片
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ab91652 (purified) at 1:30 dilution (2µg) immunoprecipitating LMO2 in Raji whole cell lysate.
Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab91652 & Raji whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 0.3 µg/ml (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse E14 embryo tissue lysates
Lane 4 : Rat E14 embryo tissue lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution (Unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab91652 被引用在 3 文献中.
- Hirano KI et al. LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice. Elife 10:N/A (2021). PubMed: 34382935
- Bergiers I et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). PubMed: 29555020
- Liu Y et al. LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction. Med Sci Monit 23:695-703 (2017). PubMed: 28170369