Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a weak, nuclear-enriched, focal staining pattern in interphase cells, which is not the expected staining pattern. MeOH-fixed samples were also evaluated and failed to produce a specific staining pattern under these conditions.
Abcam response
Thank you for your valuable feedback. Your data suggests that ab24447 may not be suitable to detect endogenous LIPG by ICC in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Nov 05 2014