Anti-LIMD1抗体(ab114104)
Key features and details
- Rabbit polyclonal to LIMD1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-LIMD1抗体
参阅全部 LIMD1 一抗 -
描述
兔多克隆抗体to LIMD1 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus -
免疫原
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).
-
阳性对照
- Whole cell lysate from HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab114104 was affinity purified using an epitope specific to LIMD1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114104于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
|
说明 |
---|
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
功能
Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
细胞定位
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli. - Information by UniProt
-
数据库链接
- Entrez Gene: 617525 Cow
- Entrez Gene: 100856566 Dog
- Entrez Gene: 8994 Human
- Entrez Gene: 29806 Mouse
- Entrez Gene: 100738579 Pig
- Omim: 604543 Human
- SwissProt: G5E5X0 Cow
- SwissProt: Q9UGP4 Human
see all -
别名
- AW822033 antibody
- D9Ertd192e antibody
- LIM domain-containing protein 1 antibody
see all
图片
-
Detection of Human LIMD1 by Western blot of Immunoprecipitate.
LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab114104 尚未被引用在任何文献中。